Part:BBa_K5036001
C-TEV
Part Description
This is known as the tobacco etch virus which is very selective for cleaving proteins at particular amino acid sequences and has been modified to have better qualities like greater heat stability, decreased self-cleavage and also allows researchers to precisely cleave the tag off, leaving behind the receptor in its unmodified form. In our model, TEV was divided into N-terminal and C-terminal fragments. So the C-terminal fragment was grafted onto the first chain of our dCas9(C)-TF-synVEGFR1 receptor.
Usage
Our dCas9(C/N)-TF-synVEGFR1/2 receptor is activated when VEGF binds to it causing (c)-TEV to bind to (N)-TEV to produce TEV protease, which cleaves TCS (Q, G) and TCS (Q, L) sites to release dcas9 from the receptor.
This figure illustrates the structure of c-terminal domain of TEV in first chain of dCas9(C)-TF-synVEGFR1 receptor. .
Dry lab Characterization
To illustrate TEV protease assembly, we used alpha fold 3 to show the interactions between its C and N domains.
Alignment Plot
3D structure of TEV protease
The alignment plot reflects that the receptor’s 3D structure has positive alignment with the experimental structures used in alpha fold 3. The results indicate favorable protein structure. Moreover, we calculated the binding stability through measuring the difference in Gibbs free energy (ΔG) and the predicted dissociation constant (M) at normal body temperature between TEV domains by the prodigy haddock tool. The ΔG was -23.7 kcal mol-1, and M=1.8e-17 which indicates a very stable and high affinity between both domains .
Characterization by Mathematical Modeling
The model provides the activation kinetics of the TEV protease which occurs subsequent to the binding of VEGF to our receptor allowing the dimerization process for our receptor chains to take place. The result shows sufficient TEV protease activation based on parametric values from literature
Graph (1). Illustrates the dimerization level (Blue line) that reaches steady state upon binding of VEGF to its receptor to activate TEV protease (Red line), The activation level of TEV protease reaches (14) to release d-Cas9 system .
Experimental Characterization
we digested dCas9(C)_NLS-Syn-VEGFR-1 grafetd with C-TEV and performed gel electrophoresis to detect the digested fragment.
This figure illustrates the digested dCas9(C)_NLS-Syn-VEGFR-1 prior to insert ligation .
Literature Characterization
This study investigated five mutations introduced into the TEV protease enzyme to improve its solubility. Following the mutations, a technique involving centrifugation was used to evaluate the solubility of each variant. The variants were concentrated ,samples were collected at specific time points, and then the protein concentrations were measured to assess their solubility.
Figure A shows how the concentration of wild-type TEV protein compares to the L56V variant. A plateau in the absorption reading indicates the point at which the protein precipitates out of solution (limited solubility). Introducing mutations L56V and S135G significantly increased TEV solubility (Figure B), allowing them to reach maximum concentrations of 6.2 mg/mL and 5.77 mg/mL, respectively. Since the remaining variants (K45F, Q58F, E106G) displayed similar solubility to the wild-type TEV, they were not investigated further.
Reference
Cabrita, L. D., Gilis, D., Robertson, A. L., Dehouck, Y., Rooman, M., & Bottomley, S. P. (2007). Enhancing the stability and solubility of TEV protease using in silico design. Protein science, 16(11), 2360-2367.
Mac Gabhann F, Popel AS. Dimerization of VEGF receptors and implications for signal transduction: a computational study. Biophys Chem. 2007 Jul;128(2-3):125-39. doi: 10.1016/j.bpc.2007.03.010. Epub 2007 Mar 24. PMID: 17442480; PMCID: PMC2711879.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 310
| None |