Part:BBa_K5024000
Uric acid oxidase
pET28a-CcxynA
BBa_K5024000 - Uric acid oxidase (UAO)
Profile
Name: Uric acid oxidase (UAO)
Base Pairs: 918bp
Origin: (Aspergillus flavus and Aspergillus niger, Synthetic)
Properties: Urate oxidase is an enzyme that catalyzes the oxidation of uric acid to allantoin. This gene has been inactivated by mutation and is nonfunctional in humans and some other primates. [provided by RefSeq, Jul 2008]. Uric acid oxidase can also be found in certain species of eukaryotic cells. For example, it is present in the kidney tissues of animals like cows and pigs.
Usage and Biology
Uric acid oxidase (UAO), also known as urate oxidase, is involved in the metabolic pathway of uric acid, which is one of the byproducts of purine metabolism. Purine metabolism generates xanthine, which is converted to uric acid through enzymatic catalysis. Uric acid oxidase plays a key role in the degradation pathway of uric acid by catalyzing its oxidation to form 5-hydroxy-2,4,6-trioxopurine (allantoin). (Figure 1)
Figure 1. The engineering schematic diagram of uric acid
Uric acid oxidase (UAO) has a variety of applications. Uric acid oxidase is commonly used in the treatment of uric acid degradation and uric acid stones. It can convert uric acid into non-soluble metabolites, helping to lower uric acid levels and reduce the formation of uric acid stones［1-3］. Relevant foreign reports have shown that gout and other complications caused by high uric acid levels can be treated by using uric acid oxidase［4－5］.
Uric acid oxidase can be used as an analytical chemical reagent to measure uric acid concentration in urine. It is of great significance for the study of uric acid metabolism and the diagnosis and monitoring of uric acid-related diseases［6－7］.
Uric acid oxidase can be used to construct biosensors for detecting and measuring the presence and concentration of uric acid. It has potential application value for monitoring the development and treatment management of uric acid-related diseases［8－9］.
Reference
[1] Rui Z, Zimai L, Yuqing S, et al. Engineered Escherichia coli Nissle 1917 with urate oxidase and an oxygen-recycling system for hyperuricemia treatment. Gut microbes, 2022, 14(1).
[2] He Yunfeng, Wang Zhiming, Fu Zhicheng, et al. Uric acid oxidase preparation and its application [P]. Chongqing: CN114438048A, 2022-05-06.
[3] Zhang Wenyu. Preparation, purification and application of a PEG-modified recombinant humanized uric acid oxidase [P]. USA: CN109852623A, 2019-06-07.
[4] Masera G, Jankovic M, Zurlo MG, Locasciulli A, Rossi MR, Cornelio Uderzo MD. Urate-oxidase prophylaxis of uric acid-induced renal damage in childhood leukemia [J]. J. Pediatric. 1982, 100:152-5.
[5] London M, Hudson PB. Uricolytic activity of purified uricase in two human beings [J]. Science, 1957, 125:937-8.
[6] Potaux L, Aparicio M, Maurel C, Ruedas ME. Therapeutique uricolytique [J]. La Nouvelle Presse Me’dicale, 1975, 4:1109-12.
[7] Altman KI, Smull K, Guzman Barron ES. A new method for the preparation of uricase and the effect of uricase on the blood uric acid levels of the chickens [J]. Arch. Biochem. Biophys, 1959, 21:115865.
[8] Ahrari S, Dabbagh F, Ahrari S, et al. Rational Design and Engineering of a Mutant Variant of Urate Oxidase as a Therapeutic Enzyme: A Molecular Dynamics Simulation Approach. Current Computer Aided-Drug Design, 2017, 13(1).
[9] Chang Yanbing, Liu Yanling, He Qiong. Pt-Au alloy nanotube modified glassy carbon electrode uricase sensor [J]. Acta Scientifica Sinica, 2013, 29(04): 515-518.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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