Part:BBa_K5022002

pET-22b(+)

pET-22b(+)

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 192
  Illegal XbaI site found at 326
  Illegal PstI site found at 4349
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 192
  Illegal PstI site found at 4349
  Illegal NotI site found at 165
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 192
  Illegal BglII site found at 392
  Illegal BamHI site found at 198
  Illegal XhoI site found at 158
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 192
  Illegal XbaI site found at 326
  Illegal PstI site found at 4349
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 192
  Illegal XbaI site found at 326
  Illegal PstI site found at 4349
  Illegal NgoMIV site found at 231
  Illegal NgoMIV site found at 424
  Illegal NgoMIV site found at 2012
  Illegal NgoMIV site found at 2172
  Illegal NgoMIV site found at 5352
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal BsaI.rc site found at 4175
  Illegal SapI site found at 3092