Part:BBa_K5007008:Design
Gal1-Kozak-CBD_syn-ADH1
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 855
Illegal BglII site found at 1317 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 150
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 2961
Illegal SapI site found at 2535
Design Notes
This device is a transcriptional unit designed specifically for S. cerevisiae. It contains the Gal1 inducible promoter with Kozak sequence and the ADH1 terminator, both quite efficient at starting and terminating the transcription of long ORFs. Under the control of the Gal1 promoter, we can induce the synthesis of olivetolic acid at specific cell densities and growth phases, allowing for optimizations in several steps. Furthermore, part BBa_K5007006 was designed as an interchangeable part, capable of being switched between plasmids and assembled with other promoter-terminator pairs, optimized for different chassis and further elaborated.
Source
Part BBa_J63006 was developed by Silver Lab and further developed and studied by several iGEM teams. The part comes from S. cerevisiae. Part BBa_K392003 was developed by iGEM10 Osaka team, with efficacy shown in different constructs.