Device

Part:BBa_K4998044:Design

Designed by: Rafail Andreou   Group: iGEM23_thessaloniki   (2023-10-10)


Kill-switch device for B. subtilis WB800N


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 2944
    Illegal suffix found in sequence at 12
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2944
    Illegal NheI site found at 2991
    Illegal NheI site found at 3014
    Illegal SpeI site found at 13
    Illegal PstI site found at 27
    Illegal NotI site found at 20
    Illegal NotI site found at 2950
    Illegal NotI site found at 4202
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2944
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 2944
    Illegal suffix found in sequence at 13
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 2944
    Illegal XbaI site found at 2959
    Illegal SpeI site found at 13
    Illegal PstI site found at 27
    Illegal NgoMIV site found at 1386
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 2184


Design Notes

Kill-switch mechanism for B. subtilis WB800N strain.


Source

LacI: BBa_K1088019, tetR: BBa_K4998041, bsrG: BBa_K4998043

References