Coding

Part:BBa_K4973006:Design

Designed by: Dimitrios Nikolaos Kapiris   Group: iGEM23_PatrasBio   (2023-10-12)


Open frame which encodes a protein with 6X His Tag consisting of mbp, sfGFP, Tachyplesin. There are


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 390
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1153


Design Notes

We wanted to make a composite part and could not find other way to add the start codon before the 6x His Tag


Source

6X His affinity Tag

References