Coding

Part:BBa_K4973006

Designed by: Dimitrios Nikolaos Kapiris   Group: iGEM23_PatrasBio   (2023-10-12)


Open frame which encodes a protein with 6X His Tag consisting of mbp, sfGFP, Tachyplesin. There are

A part with ATG codon and 6X His Tag, so that being able to put a coding sequwncw without start codon after the His Tag.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 390
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1153


[edit]
Categories
Parameters
None