Coding

Part:BBa_K4973001:Design

Designed by: Dimitrios Nikolaos Kapiris   Group: iGEM23_PatrasBio   (2023-10-07)


Y8S-> Y8S Analog of Tachyplesin gene


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Tacchyplesin has hemolytic action, so we will try to produce its analog Y8S with very limited hemolytic action.

Source

We found the aminoacid sequence at (Panteleev PV, Ovchinnikova TV, 2017) and reverse translated to nucleotide sequence at SnapGene.

References

Panteleev PV, Ovchinnikova TV. Improved strategy for recombinant production and purification of antimicrobial peptide tachyplesin I and its analogs with high cell selectivity. Biotechnol Appl Biochem. 2017 Jan;64(1):35-42. doi: 10.1002/bab.1456. Epub 2015 Dec 22. PMID: 26549611.