Part:BBa_K4971006:Design
RNA sequence containing gRNA and scaffold RNA traget to mutate Cas1 protein in K.12 E.coli
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Source
The whole sequence of Cas5 protein's CDS is from NCBI database, and the sequence is shown below. ATGACCTGGCTTCCCCTTAATCCCATTCCACTCAAAGATCGCGTCTCCATGATCTTTCTGCAATATGGGCAGATCGATGTAATAGATGGCGCGTTTGTACTTATCGACAAGACAGGGATCCGCACTCATATTCCTGTTGGCTCGGTTGCCTGCATCATGCTGGAACCTGGTACACGGGTTTCGCATGCAGCTGTACGCCTGGCTGCGCAAGTTGGAACATTGTTGGTATGGGTGGGGGAAGCGGGCGTTCGTGTTTATGCTTCTGGTCAGCCTGGAGGTGCGCGTTCAGATAAGCTGCTCTATCAGGCAAAACTTGCTCTGGATGAAGATTTGCGTCTGAAGGTCGTACGTAAAATGTTTGAACTTCGGTTTGGAGAACCTGCGCCTGCCCGGCGCTCCGTAGAGCAACTCAGAGGTATAGAAGGCAGTCGCGTGCGGGCAACCTACGCACTTCTGGCGAAGCAATACGGCGTGACATGGAATGGACGTCGCTACGATCCGAAAGACTGGGAAAAGGGCGATACGATCAACCAATGCATTAGCGCTGCAACTTCCTGTTTATACGGCGTAACTGAAGCGGCGATACTTGCAGCTGGTTATGCACCAGCTATTGGGTTTGTGCATACAGGAAAGCCTCTTTCCTTTGTTTACGATATTGCAGACATCATTAAATTTGACACTGTTGTACCGAAAGCTTTTGAGATAGCGCGTCGTAACCCTGGTGAGCCGGACCGGGAAGTCCGTTTGGCGTGCAGGGATATTTTTCGCAGTAGTAAAACATTAGCCAAATTGATTCCGCTTATAGAGGACGTGCTTGCCGCTGGAGAAATACAACCGCCGGCCCCACCTGAAGATGCACAGCCTGTTGCCATTCCGCTTCCTGTTTCACTGGGAGATGCAGGCCATCGGAGTAGCTGA The scaffold sequence if from "Optimized CRISPR guide RNA design for two high-fidelity Cas9 variants by deep learning", Daqi Wang et al. The sequence is shown below. Gttttagagctagaaatagcaagttaaaataaggctagtccgttatcaacttgaaaaagtggcaccgagtcggtgcttttt