Translational_Unit

Part:BBa_K497003

Designed by: Joanna Hare   Group: iGEM10_Macquarie_Australia   (2010-10-24)

Deinococcus radiodurans bacteriophytochrome gene with Ribosome Binding Site

The Deinococcus radiodurans bacteriophytochrome binds to biliverdin, a chromophore. Biliverdin is produced in the heme metabolic pathway catalysed by heme oxygenase. Once bound to the chromophore the complex can absorb red and far red light causing the complex to change colour from blue to green reversibly. This part also has a ribosome binding site (AGGAGG) located 6 base pairs upstream from the ATG start codon. Sequence from National Center for Biotechnology

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 385
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 385
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 385
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 385
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 385
    Illegal NgoMIV site found at 1252
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1682


[edit]
Categories
Parameters
None