Coding

Part:BBa_K497001:Design

Designed by: Joanna Hare   Group: iGEM10_Macquarie_Australia   (2010-10-23)

Deinococcus radiodurans bacteriophytochrome gene


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 373
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 373
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 373
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 373
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 373
    Illegal NgoMIV site found at 1240
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1670


Design Notes

The D. radiodurans bacteriophytochrome includes an internal EcoR1 site which creates a problem for Biobrick construction and submission as a DNA part following standard submission protocol.



Source

Deinococcus radiodurans genome. National Center for Biotechnology Information

References