Composite

Part:BBa_K4968019:Design

Designed by: Shouye Zhu   Group: iGEM23_XJTLU-CHINA   (2023-10-09)


SUMO-SmtA-CBM-sfGFP


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 206
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 206
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 206
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 206
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 206
    Illegal AgeI site found at 988
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 81
    Illegal BsaI site found at 594
    Illegal BsaI.rc site found at 587


Source

The sequence of SUMO tag is from pET21a-SUMO. The SmtA sequence is derived from NCBI. The sequence of the family 2 CBM from endoglucanase A of C.fimi was obtained from NCBI. The sfGFP sequence is derived from the Snapgene database. Linker between SUMO and SmtA: GSGSGS. Linker between SmtA and CBM: GSGGAGGS. Linker between CBM and sfGFP: GGGSPTGG.

References

Aïssa, K. et al. (2019) ‘Functionalizing cellulose nanocrystals with click modifiable carbohydrate-binding modules’, Biomacromolecules, 20(8), pp. 3087–3093. doi:10.1021/acs.biomac.9b00646.

Blindauer, C.A. (2011) ‘Bacterial metallothioneins: Past, present, and questions for the future’, JBIC Journal of Biological Inorganic Chemistry, 16(7), pp. 1011–1024. doi:10.1007/s00775-011-0790-y.

Blindauer, C.A. et al. (2007) ‘Histidine ligands in bacterial metallothionein enhance cluster stability’, JBIC Journal of Biological Inorganic Chemistry, 12(3), pp. 393–405. doi:10.1007/s00775-006-0196-4.

Chang, H.-M. and Yeh, E.T. (2020) ‘Sumo: From bench to bedside’, Physiological Reviews, 100(4), pp. 1599–1619. doi:10.1152/physrev.00025.2019.

Feng, N., Jiang, F.J. and Chun, C.H. (2017) ‘Metallothionein and Their Biological Functions’, Chinese Journal of Biochemistry and molecular Biology, 33(9), pp. 893–899. doi:10.13865/j.cnki.cjbmb.2017.09.07.

Oliveira, N.M. et al. (2015) ‘Biofilm Formation as a response to ecological competition’, PLOS Biology, 13(7). doi:10.1371/journal.pbio.1002191.

Pédelacq, J.-D. et al. (2005) ‘Engineering and characterization of a superfolder green fluorescent protein’, Nature Biotechnology, 24(1), pp. 79–88. doi:10.1038/nbt1172.

Vertegaal, A.C. (2022) ‘Signalling mechanisms and cellular functions of Sumo’, Nature Reviews Molecular Cell Biology, 23(11), pp. 715–731. doi:10.1038/s41580-022-00500-y.