Composite

Part:BBa_K4968017:Design

Designed by: Shouye Zhu   Group: iGEM23_XJTLU-CHINA   (2023-10-09)


CBM-sfGFP-pUC_GW_KanR-SUMO


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 3885
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3885
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3885
    Illegal XhoI site found at 1078
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 3885
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 3885
    Illegal AgeI site found at 487
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 93
    Illegal BsaI site found at 3760
    Illegal BsaI.rc site found at 86


Source

The segment CBM-sfGFP-pUC_GW_KanR-SUMO was obtained from the pUC_GW_Kan-SUMO-SmtA-CBM-sfGFP plasmid using PCR technology with Primer 1 (AGATCCTGATCCAGAGCCGC) and Primer 2 (GCACCAGGTTGTCGTGTAGACT). Linker between CBM and sfGFP: GGGSPTGG.

References