Plasmid_Backbone

Part:BBa_K4961001:Design

Designed by: Liu Yue   Group: iGEM23_YangzhouNOFLS   (2023-10-07)


PEE6.4


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal XbaI site found at 2523
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BglII site found at 6975
    Illegal XhoI site found at 1790
    Illegal XhoI site found at 2505
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal XbaI site found at 2523
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal XbaI site found at 2523
    Illegal NgoMIV site found at 2955
    Illegal NgoMIV site found at 4296
    Illegal NgoMIV site found at 4579
    Illegal AgeI site found at 795
    Illegal AgeI site found at 2493
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI.rc site found at 759
    Illegal BsaI.rc site found at 6107
    Illegal SapI site found at 5024
    Illegal SapI.rc site found at 2179
    Illegal SapI.rc site found at 4145
    Illegal SapI.rc site found at 4355


Design Notes

No


Source

Commercial plasmids produced by biological companies

References