Project

Part:BBa_K4955000:Design

Designed by: Kaisei Otake   Group: iGEM23_Japan-United   (2023-09-22)


Enzymes involved in Crocetin biosynthesis


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1643
    Illegal PstI site found at 5570
    Illegal PstI site found at 5981
    Illegal PstI site found at 8833
    Illegal PstI site found at 8951
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1643
    Illegal PstI site found at 5570
    Illegal PstI site found at 5981
    Illegal PstI site found at 8833
    Illegal PstI site found at 8951
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 6388
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1643
    Illegal PstI site found at 5570
    Illegal PstI site found at 5981
    Illegal PstI site found at 8833
    Illegal PstI site found at 8951
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1643
    Illegal PstI site found at 5570
    Illegal PstI site found at 5981
    Illegal PstI site found at 8833
    Illegal PstI site found at 8951
    Illegal NgoMIV site found at 6712
    Illegal NgoMIV site found at 6842
    Illegal NgoMIV site found at 8638
    Illegal NgoMIV site found at 8695
    Illegal AgeI site found at 991
    Illegal AgeI site found at 2015
    Illegal AgeI site found at 2148
    Illegal AgeI site found at 2555
    Illegal AgeI site found at 7616
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2493
    Illegal BsaI.rc site found at 2927
    Illegal SapI site found at 1648


Design Notes

aaa


Source

b

References