Coding

Part:BBa_K4944001:Design

Designed by: Giovanna Acosta   Group: iGEM23_Calgary   (2023-10-12)


hNPC1L1


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 3253
    Illegal PstI site found at 253
    Illegal PstI site found at 378
    Illegal PstI site found at 1372
    Illegal PstI site found at 1432
    Illegal PstI site found at 1504
    Illegal PstI site found at 2128
    Illegal PstI site found at 2389
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 253
    Illegal PstI site found at 378
    Illegal PstI site found at 1372
    Illegal PstI site found at 1432
    Illegal PstI site found at 1504
    Illegal PstI site found at 2128
    Illegal PstI site found at 2389
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 260
    Illegal BglII site found at 3821
    Illegal BamHI site found at 610
    Illegal BamHI site found at 880
    Illegal BamHI site found at 984
    Illegal BamHI site found at 4051
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 3253
    Illegal PstI site found at 253
    Illegal PstI site found at 378
    Illegal PstI site found at 1372
    Illegal PstI site found at 1432
    Illegal PstI site found at 1504
    Illegal PstI site found at 2128
    Illegal PstI site found at 2389
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 3253
    Illegal PstI site found at 253
    Illegal PstI site found at 378
    Illegal PstI site found at 1372
    Illegal PstI site found at 1432
    Illegal PstI site found at 1504
    Illegal PstI site found at 2128
    Illegal PstI site found at 2389
    Illegal NgoMIV site found at 1855
    Illegal NgoMIV site found at 2773
    Illegal NgoMIV site found at 2800
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Enter any design considerations you had to deal with during the detailed design of the sequence.


Source

Sourced from AddGENE plasmid. From Giulio Superti-Furga Lab and RESOLUTE consortium SLC collection (Addgene plasmid # 161469 ; http://n2t.net/addgene:161469; RRID:Addgene_161469)


References