Coding
Part:BBa_K4944001:Design
Designed by: Giovanna Acosta Group: iGEM23_Calgary (2023-10-12)
hNPC1L1
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 3253
Illegal PstI site found at 253
Illegal PstI site found at 378
Illegal PstI site found at 1372
Illegal PstI site found at 1432
Illegal PstI site found at 1504
Illegal PstI site found at 2128
Illegal PstI site found at 2389 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 253
Illegal PstI site found at 378
Illegal PstI site found at 1372
Illegal PstI site found at 1432
Illegal PstI site found at 1504
Illegal PstI site found at 2128
Illegal PstI site found at 2389 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 260
Illegal BglII site found at 3821
Illegal BamHI site found at 610
Illegal BamHI site found at 880
Illegal BamHI site found at 984
Illegal BamHI site found at 4051 - 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 3253
Illegal PstI site found at 253
Illegal PstI site found at 378
Illegal PstI site found at 1372
Illegal PstI site found at 1432
Illegal PstI site found at 1504
Illegal PstI site found at 2128
Illegal PstI site found at 2389 - 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 3253
Illegal PstI site found at 253
Illegal PstI site found at 378
Illegal PstI site found at 1372
Illegal PstI site found at 1432
Illegal PstI site found at 1504
Illegal PstI site found at 2128
Illegal PstI site found at 2389
Illegal NgoMIV site found at 1855
Illegal NgoMIV site found at 2773
Illegal NgoMIV site found at 2800 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Enter any design considerations you had to deal with during the detailed design of the sequence.
Source
Sourced from AddGENE plasmid. From Giulio Superti-Furga Lab and RESOLUTE consortium SLC collection (Addgene plasmid # 161469 ; http://n2t.net/addgene:161469; RRID:Addgene_161469)