Composite

Part:BBa_K4941106:Design

Designed by: Xinyu Chen   Group: iGEM23_ZJFH-Nanjing   (2023-10-12)


ku70UP-ylER-LhYida-URA-ku70dw


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 6666
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 3611
    Illegal PstI site found at 6666
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1553
    Illegal XhoI site found at 1951
    Illegal XhoI site found at 5083
    Illegal XhoI site found at 5116
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 6666
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 6666
    Illegal NgoMIV site found at 5271
    Illegal AgeI site found at 2430
    Illegal AgeI site found at 3989
    Illegal AgeI site found at 4213
    Illegal AgeI site found at 6041
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1399
    Illegal BsaI.rc site found at 814
    Illegal BsaI.rc site found at 2753
    Illegal BsaI.rc site found at 4536
    Illegal SapI site found at 1701
    Illegal SapI.rc site found at 522


Design Notes

none


Source

yarrowia lipolytica

References