Plasmid

Part:BBa_K4941041:Design

Designed by: Xinyu Chen   Group: iGEM23_ZJFH-Nanjing   (2023-10-08)


pylxp-CEN1-1-pTEF-Incomplete Intron-LhYida-XPR2_terminator -ORI1001-Rep Origin (pMB1)-AmpR-Leu2 mark


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1095
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 4857
    Illegal XhoI site found at 4886
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 5743
    Illegal AgeI site found at 1473
    Illegal AgeI site found at 1697
    Illegal AgeI site found at 4272
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3451
    Illegal BsaI site found at 5028
    Illegal BsaI.rc site found at 237
    Illegal BsaI.rc site found at 4943
    Illegal BsaI.rc site found at 5226
    Illegal SapI.rc site found at 4474


Design Notes

none


Source

Lactobacillus helveticus

References