Plasmid
Part:BBa_K4941035:Design
Designed by: Xinyu Chen Group: iGEM23_ZJFH-Nanjing (2023-10-08)
pet28a- T7 promoter- DnXR- His tag- T7 terminator- f1 origin- ColE1 pBR322 origin- kan-lac I
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 576
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 576
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 576
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 576
Illegal NgoMIV site found at 1201 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
none
Source
Debaryomyces nepalensis NCYC 3413 and Escherichia coli