![](https://parts.igem.org/images/partbypart/icon_composite.png)
Composite
Part:BBa_K4940011:Design
Designed by: Kuang Minjie Group: iGEM23_iZJU-China (2023-10-11)
Transcription unit of P450BM-3 QM-his and GlcDH-II-his
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1441
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1441
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1441
Illegal XhoI site found at 1952 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1441
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1441
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 1287
Design Notes
The main idea was to have a high expression of the gene and the expression should be controlled safely. In that case, commercial T7 promoter is useful and lac operator represses the gene expression without IPTG induction.
Source
Most of the parts are already in the commercial plasmids, and we select a proper vector to use. The gene sequence was found in NCBI and synthesized by the company.