![](https://parts.igem.org/images/partbypart/icon_composite.png)
Composite
Part:BBa_K4940010:Design
Designed by: Kuang Minjie Group: iGEM23_iZJU-China (2023-10-11)
Transcription unit of Prα-POL-his
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 140
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 346
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The main idea was to have a high expression of the gene and the expression should be controlled safely. In that case, commercial T7 promoter is useful and lac operator represses the gene expression without IPTG induction.
Source
Most of the parts are already in the commercial plasmids, and we select a proper vector to use. The gene sequence was found in NCBI and synthesized by the company.