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Part:BBa_K494000:Design
Malachite Green Binding Aptamer
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1
Illegal BamHI site found at 33 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Sequence was ordered by Biomers. Originally ordered Sequence: aggctcctaatacgactcactatag ggagaattcgcggccgcttctagag ggatcccgactggcgagagccaggt aacgaatggatcctactagtagcgg ccgctgcag
Amplified by PCR, cut with EcoRI and PstI and cloned into precut, linearized pSB1C3.
Source
Synthetic aptamer evolved using SELEX. First described in Grate, D. and C. Wilson, Laser-mediated, site-specific inactivation of RNA transcripts. Proceedings of the National Academy of Sciences of the United States of America, 1999. 96(11): p. 6131.
References
Design:
Grate, D. and C. Wilson, Laser-mediated, site-specific inactivation of RNA transcripts. Proceedings of the National Academy of Sciences of the United States of America, 1999. 96(11): p. 6131.
Spectral properties:
Babendure, J.R., S.R. Adams, and R.Y. Tsien, Aptamers switch on fluorescence of triphenylmethane dyes. J. Am. Chem. Soc, 2003. 125(48): p. 14716-14717.
Threedimensional Structure:
Baugh, C., D. Grate, and C. Wilson, 2.8 Ã… crystal structure of the malachite green aptamer1. Journal of Molecular Biology, 2000. 301(1): p. 117-128.
Applications:
tojanovic, M.N. and D.M. Kolpashchikov, Modular aptameric sensors. J. Am. Chem. Soc, 2004. 126(30): p. 9266-9270.
Stead, S.L., et al., An RNA-Aptamer-Based Assay for the Detection and Analysis of Malachite Green and Leucomalachite Green Residues in Fish Tissue. Analytical chemistry. 82(7): p. 2652-2660.
Weigand, J.E. and B. Suess, Aptamers and riboswitches: perspectives in biotechnology. Applied microbiology and biotechnology, 2009. 85(2): p. 229-236.