Coding

Part:BBa_K4940003:Design

Designed by: Kuang Minjie   Group: iGEM23_iZJU-China   (2023-10-10)


Prα-POL-his


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 71
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 277
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Since the gene has a clear sequence and has been proven useful based on many experiments, no certain considerations were in the design process. A his tag was fused to the protein for further purification.

Source

This gene has been sequenced from Pseudomonas rhodesiae  CIP107491 (1).

References

1. Dubessay P, Larroche C, Fontanille P. Cloning and Characterization of the Gene Encoding Alpha-Pinene Oxide Lyase Enzyme (Prα-POL) from Pseudomonas rhodesiae CIP 107491 and Production of the Recombinant Protein in Escherichia coli. Appl Biochem Biotechnol. 2018 Jul;185(3):676–90.