![](https://parts.igem.org/images/partbypart/icon_coding.png)
Coding
Part:BBa_K4940003:Design
Designed by: Kuang Minjie Group: iGEM23_iZJU-China (2023-10-10)
Prα-POL-his
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 71
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 277
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Since the gene has a clear sequence and has been proven useful based on many experiments, no certain considerations were in the design process. A his tag was fused to the protein for further purification.
Source
This gene has been sequenced from Pseudomonas rhodesiae CIP107491 (1).
References
1. Dubessay P, Larroche C, Fontanille P. Cloning and Characterization of the Gene Encoding Alpha-Pinene Oxide Lyase Enzyme (Prα-POL) from Pseudomonas rhodesiae CIP 107491 and Production of the Recombinant Protein in Escherichia coli. Appl Biochem Biotechnol. 2018 Jul;185(3):676–90.