Composite

Part:BBa_K4937020:Design

Designed by: Chen Wang   Group: iGEM23_SCU-BSE-China   (2023-09-27)


OAZ1_upstream-TEF1p-SPE1-PRM9t-TDH3p-AtACL5-DIT1t-OAZ1_downstream


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2336
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2072
    Illegal BglII site found at 5129
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 632


Design Notes

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Source

This part is from fusion PCR of BBa_K4937016, BBa_K4937000, BBa_K4937012, BBa_K4937005, BBa_K4937009, BBa_K4937001, BBa_K4937012, BBa_K4937008, BBa_K4937010, and BBa_K4937017.

References

Qin, J., Krivoruchko, A., Ji, B. et al. Engineering yeast metabolism for the discovery and production of polyamines and polyamine analogues. Nat Catal 4, 498–509 (2021). https://doi.org/10.1038/s41929-021-00631-z

Hamilton, R., Watanabe, C. K., & de Boer, H. A. (1987). Compilation and comparison of the sequence context around the AUG startcodons in Saccharomyces cerevisiae mRNAs. Nucleic acids research, 15(8), 3581–3593. https://doi.org/10.1093/nar/15.8.3581