![](https://parts.igem.org/images/partbypart/icon_dna.png)
Part:BBa_K4933002:Design
DNAzyme FBS1
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 23
Templates
Double Stranded Template to maintain stock (Will be used to generate DNAzyme and Compliment sequence of DNAzyme):
CTGAAGAGTTGATGGTAGGTATGCTCTTCAGCGATGACTGATATTCTCTA
AACCAGCGCAGTCCACCCATGTTAGTGACCGTTATAATTCCCGTCCCTCA
CTAACGTTCGGTAACGGAGAAACTCTGCGGCATACTTTATTATATATATG
AAACGTGCCCAAGTGATGCTAAACAAGTCTTAGCTGGTTCCTGTGTTAGT
TTAAGGGTAAACATACAAGTTGATTGAACATGGGTTGGGGGCTTCAAATC
ATCGAAGACTCTATAGTATCTCGAAGACCAAGTAGGGCAGCCTATAGTTT
P.S. Single stranded template compliment to DNAzyme was also ordered and used
Primers for PCR
Asymmetric to obtain DNAzyme sequence:
GTTATAATTCCCGTCCCTCACTAAC
Tm = 54.9°C
Asymmetric to obtain compliment DNAzyme sequence:
GTTAGTGAGGGACGGGAATTATAACGG
Tm = 58.5°C
To obtain DNAzyme sequence from ssDNA template:
CTGAAGAGTTGATGGTAGGTATGCTCT
Tm = 57.7°C
ReqLoop Sequence Identities
CoVID specific sequence (X parameter): TTGATGGTAGGTATAATT
Omicron specific sequence (Y parameter): ATTCTCTAAACCAGCG
Delta specific sequence (Z parameter): TTATAATTCCCGT
References
[1] Genome Sequences of the Delta Variant (B.1.617.2) and the Kappa Variant (B.1.617.1) Detected in Morocco: https://doi.org/10.1128%2FMRA.00727-21 accession numbers MZ208926 and MZ571142
[2] SARS-CoV-2 B.1.1.529 (Omicron) Variant — United States, December 1–8, 2021: https://doi.org/10.15585%2Fmmwr.mm7050e1
[3] SARS-CoV-2 genome sequences:
GISAID database identifiers: EPI_ISL_2110643 and EPI_ISL_2966236
NCBI GeneBank Accession numbers: MZ208926 and MZ571142
[4] Analysis Tool Web Services from the EMBL-EBI. (2013) McWilliam H, Li W, Uludag M, Squizzato S, Park YM, Buso N, Cowley AP, Lopez R Nucleic acids research 2013 Jul;41(Web Server issue):W597-600 doi:10.1093/nar/gkt376