Composite

Part:BBa_K4927057:Design

Designed by: Chuan Liu   Group: iGEM23_HUBU-SKY-China   (2023-10-08)


HydABCC


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

You need to pay attention to the location of the CL9 tag, you can choose to attach it to the C and N terminals of any of the subunits, but you need to test because different connection locations will cause HydABCC to exhibit different activity.


Source

The NADH-dependent [Fe-Fe] hydrogenase selected in this study was derived from Trophomonas wolfei.

References