Composite
A2
Part:BBa_K4927044:Design
Designed by: Chuan Liu Group: iGEM23_HUBU-SKY-China (2023-10-06)
A2
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
You need to note that the label is connected to the N-terminal or C-terminal of methanol dehydrogenase, as this can cause changes in the activity of methanol dehydrogenase.After our testing, we found that when the label is connected to the C-terminal, the activity of methanol dehydrogenase is better.
Source
Methanol dehydrogenase is derived from Stearothermophilus, and the CL2 label was designed by our team.