Part:BBa_K4927010
IM8
Introduction
Our team designed truncated mutants CL7, which were inactivated by E. coli CE7 nuclease. Based on the high similarity of amino acid sequences between CE2, CE8, and CE9 and CE7, corresponding CE2, CE8, and CE9 truncated mutants CL2, CL8, and CL9 were designed, and corresponding inhibitors Im2, Im7, Im8, and Im9 were obtained to construct an artificial protein scaffold Scaf-CIQ
Usage and Biology
We need to use IM2, IM7, IM8, IM9, and three different Linkers to form a complete protein scaffold. IM8 is an important component of the protein scaffold.
Figure1:The strcture of protein scaffold
Rerference
[1]Yang J, Wang F, Yang S, et al. Development of a Hyperthermostable Artificial Scaffold Based on Ultrahigh-Affinity Protein Pairs and Its Application in Cellulose Degradation [J]. ACS Sustainable Chemistry & Engineering, 2022, 10(6): 2072-83.
[2]Touahar, I. E.; Haroune, L.; Ba, S.; Bellenger, J.-P.; Cabana, H. Characterization of combined cross-linked enzyme aggregates from laccase, versatile peroxidase and glucose oxidase, and their utilization for the elimination of pharmaceuticals. Sci. Total Environ. 2014, 481, 90−99.
[3]Zhang, Y.; Yong, Y.; Ge, J.; Liu, Z. Lectin Agglutinated Multienzyme Catalyst with Enhanced Substrate Affinity and Activity. ACS Catal. 2016, 6, 3789−3795.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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