Coding
CL8-tag

Part:BBa_K4927006

Designed by: Chuan Liu   Group: iGEM23_HUBU-SKY-China   (2023-10-06)


CL8

Introduction

Our team designed truncated mutants CL7, which were inactivated by E. coli CE7 nuclease. Based on the high similarity of amino acid sequences between CE2, CE8, and CE9 and CE7, corresponding CE2, CE8, and CE9 truncated mutants CL2, CL8, and CL9 were designed, and corresponding inhibitors Im2, Im7, Im8, and Im9 were obtained to construct an artificial protein scaffold Scaf-CIQ

Figure1:a.CE7/IM7 reaction pairs; B.CE7/DNA reaction pair;C.CE7/CL7 protein sequence alignment

Usage and Biology

The CL7 tag will bind to formic dehydrogenase (FDH)） to form complex M8 and 8F(See the figure below）

Figure2:The strcture of M8


Figure3:The strcture of 8F


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None