Part:BBa_K4912001
Two component flavin dependent monooxygenase
4-hydroxyphenylacetic acid-3-monooxygenase (HpaBC) is widely present in various microorganisms and undergoes hydroxylation reactions on various aromatic compounds, including tyrosol, naringin, p-coumaric acid, caffeic acid, 3,4-dihydroxyphenylpyruvate, ferulic acid, and coninal. HpaBC is a two component flavin dependent monooxygenase that uses FMN or FAD reduced by reductase for molecular oxygen activation. These two components are generally encoded adjacent to each other on the genome.It can catalyze the ortho hydroxylation of 4HPA. HpaB is a FADH2 dependent monooxygenase, while HPAC is a flavin dependent oxidoreductase that supplies FADH/FMNH- to HpaB Studies have shown that HpaBC from Escherichia coli has strong catalytic ability for tyrosol and is used to synthesize hydroxytyrosol. Therefore, in this study, we selected HpaBC encoding hydroxylase from Escherichia coli
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 67
Illegal PstI site found at 1408 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 67
Illegal PstI site found at 1408 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 83
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 67
Illegal PstI site found at 1408 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 67
Illegal PstI site found at 1408 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 1863
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