Composite

Part:BBa_K4902008:Design

Designed by: Liam Davidsson   Group: iGEM23_Chalmers-Gothenburg   (2023-10-10)


FMN -> alpha-ribazole


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 912
    Illegal EcoRI site found at 3117
    Illegal SpeI site found at 4060
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 912
    Illegal EcoRI site found at 3117
    Illegal SpeI site found at 4060
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 912
    Illegal EcoRI site found at 3117
    Illegal BamHI site found at 2982
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 912
    Illegal EcoRI site found at 3117
    Illegal SpeI site found at 4060
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 912
    Illegal EcoRI site found at 3117
    Illegal SpeI site found at 4060
    Illegal AgeI site found at 2379
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The part was designed to be ordered as three segments of roughly equal size and then combined via Gibson assembly without scars. The parts themselves were ordered without overhangs but instead relied on vector specific primers to add the overlap, which allowed for more flexibility.

Source

The part is made up of several iGEM parts, most of which are created by the team, with promotors and terminators from other teams. The parts we created are BBa_K4902001-2007. Parts BBa_K3183000 (eryth promoter) and BBa_K3183021 (double terminator) were created by iGEM19_Oxford. The part BBa_K2978207 (CP44 promoter) was created by iGEM19_diffEASY.

References