Coding
h
Part:BBa_K4899006:Design
Designed by: Morten Pedersen Hauger Group: iGEM23_UiOslo-Norway (2023-10-11)
His-tagged dead cas12f, CasMINI
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1417
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1417
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1417
Illegal BglII site found at 350
Illegal BglII site found at 359
Illegal BglII site found at 428 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1417
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1417
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
modeling to ensure tag does not interfere with binding and dimer formation
Source
literture, and own design