Coding
Part:BBa_K4899005:Design
Designed by: Morten Pedersen Hauger Group: iGEM23_UiOslo-Norway (2023-10-11)
strep tagged dcas12f (dcasMINI)
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1354
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1354
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1354
Illegal BglII site found at 287
Illegal BglII site found at 296
Illegal BglII site found at 365 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1354
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1354
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
ensure that dimer formation was not inhibited by addition of tags
Source
modifed version of BBa_K4899004, based on lieterture