DNA

Part:BBa_K4897001:Design

Designed by: Ji Zhiyuan   Group: iGEM23_BS-United-China   (2023-10-03)


BS DNA-162 (BS DNA 2.0) using in L-RCA for detecting P. acne


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 83
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 57
    Illegal SpeI site found at 83
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 68
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 83
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 83
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Normal Taq DNA polymerase may not appropriate in this DNA rolling cycle amplification chain reaction.


Source

the binding region is specific binding to P. acne 16S rRNA gene

References