DNA
Part:BBa_K4897001:Design
Designed by: Ji Zhiyuan Group: iGEM23_BS-United-China (2023-10-03)
BS DNA-162 (BS DNA 2.0) using in L-RCA for detecting P. acne
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 83
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 57
Illegal SpeI site found at 83 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 68
- 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 83
- 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 83
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Normal Taq DNA polymerase may not appropriate in this DNA rolling cycle amplification chain reaction.
Source
the binding region is specific binding to P. acne 16S rRNA gene