DNA
PQQGDH
Part:BBa_K4893001:Experience
Designed by: Corinne Drabenstott Group: iGEM23_UNC-ChapelHill (2023-10-07)
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We tested to determine whether pyocyanin could be an electron acceptor of the PQQGDH and glucose oxidation reaction for the purpose of a pyocyanin biosensor. The protein was successfully expressed by Escherichia coli BL21 DE3 cells and tested with PQQGDH, although results were inconclusive and require further activity testing. One recommendation is to incubate PQQGDH with Ca2+ ions to improve the metal-ion coordinate binding between the GDH and PQQ cofactor.
Applications of BBa_K4893001
Glucose sensors, enzyme fuel cells
User Reviews
UNIQ6ffc1732cc8de67d-partinfo-00000000-QINU UNIQ6ffc1732cc8de67d-partinfo-00000001-QINU