DNA
PQQGDH

Part:BBa_K4893001:Design

Designed by: Corinne Drabenstott   Group: iGEM23_UNC-ChapelHill   (2023-10-07)


Pyrroloquinoline Quinone Glucose Dehydrogenase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 450
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The replacement of Ser231 to Lys resulted in an increase in thermal stability.[1]

Source

Acinetobacter calcoaceticus

References

[1] Sode K, Ootera T, Shirahane M, Witarto AB, Igarashi S, Yoshida H. Increasing the thermal stability of the water-soluble pyrroloquinoline quinone glucose dehydrogenase by single amino acid replacement. Enzyme Microb Technol. 2000 Apr 1;26(7):491-496. doi: 10.1016/s0141-0229(99)00196-9. PMID: 10771051.

[2] Igarashi S, Sode K. Stabilization of quaternary structure of water-soluble quinoprotein glucose dehydrogenase. Mol Biotechnol. 2003 Jun;24(2):97-104. doi: 10.1385/MB:24:2:97. PMID: 12746550.