DNA

Part:BBa_K4890004:Design

Designed by: Duoqing LIN   Group: iGEM23_Shanghai-SDG   (2023-08-14)


Hsp70


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 141
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 141
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 141
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 141
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We used pUAST vector system to construct pUAST-MTF-1 plasmid. pUAST vector contains UAS and Hsp70. We inserted MTF-1 gene in the pUAST vector downstream of UAS and Hsp70 by enzyme cutting and linking, and constructed pUAST-MTF-1 plasmid.


Source

The source of the part will be its sequence which was retrieved from pUAST sequence.


References