DNA

Part:BBa_K4890002:Design

Designed by: Duoqing LIN   Group: iGEM23_Shanghai-SDG   (2023-08-14)


MRE


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We aim to construct Drosophila lines to detect heavy metals in the environment. MTF-1 is used to trigger the response of Drosophila to heavy metal ions. MTF-1 is a transcription factor that can be activated by heavy metals. In the cell nucleus of Drosophila cells, MTF-1 binds to MRE to recruit RNA polymerase, in turn increasing the expression of Mto/Mtn to detoxicate metals. We replaced Mto/Mtn with Hid (head involution defective) to visualize transcription process.


Source

The source of the part will be its sequence which was retrieved from published article (Silar, P., L. Theodore, R. Mokdad, N. E. Erraiss, A. Cadic, and M. Wegnez.1990. Metallothionein Mto gene of Drosophila melanogaster: structure and regulation. J. Mol. Biol. 215:217–224.).


References