DNA
Part:BBa_K4889000:Design
Designed by: zhaoxia zhang Group: iGEM23_Shanghai-MedX (2023-08-24)
MST2 1-308
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 186
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 12
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 863
Design Notes
The Hippo pathway has been identified as a potential drug target for cancer. Furthermore, the MST2-STRN3 interaction was chosen as a target because of its importance in the Hippo pathway and previous studies in the laboratory. In this project, we want to find out if the FDA drug can disrupt the MST2-STRN3 interaction to activate the Hippo pathway. First, we need to express these proteins.
Source
MST2 1-308 from Homo sapiens are synthesized.