Coding

Part:BBa_K4888002:Design

Designed by: Cassandre Pitz   Group: iGEM23_EPFL   (2023-09-19)


SpaC, mucus-binding protein


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1831
    Illegal PstI site found at 1871
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1534
    Illegal PstI site found at 1831
    Illegal PstI site found at 1871
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 742
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1831
    Illegal PstI site found at 1871
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1831
    Illegal PstI site found at 1871
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Shortening of the original SpaC sequence


Source

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2746127/

References