Coding
Part:BBa_K4888002:Design
Designed by: Cassandre Pitz Group: iGEM23_EPFL (2023-09-19)
SpaC, mucus-binding protein
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 1831
Illegal PstI site found at 1871 - 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1534
Illegal PstI site found at 1831
Illegal PstI site found at 1871 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 742
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 1831
Illegal PstI site found at 1871 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 1831
Illegal PstI site found at 1871 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Shortening of the original SpaC sequence
Source
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2746127/