DNA

Part:BBa_K4887003:Design

Designed by: Duoqing LIN   Group: iGEM23_Shanghai-BioX   (2023-08-14)


3*FLAG


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

It is the FLAG geng harboured in the backbone of plasmid psgR-Cas9-At.The transgenic lines can be detected by western blot using FLAG antibody.


Source

3*FLAG is synthesized according to Wenshan Liu et al. (2015)


References

Wenshan Liu, Xiaohong Zhu, Mingguang Lei, Qingyou Xia, JR Botella, Jian-Kang Zhu, Yanfei Mao. A detailed procedure for CRISPR/Cas9-mediated gene editing in Arabidopsis. Science Bulletin, 2015, 15(60).