Part:BBa_K4886007:Design
F/Xpk(ASR)
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 779
Illegal PstI site found at 1334
Illegal PstI site found at 1928
Illegal PstI site found at 1964 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 779
Illegal PstI site found at 1334
Illegal PstI site found at 1928
Illegal PstI site found at 1964 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 779
Illegal PstI site found at 1334
Illegal PstI site found at 1928
Illegal PstI site found at 1964 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 779
Illegal PstI site found at 1334
Illegal PstI site found at 1928
Illegal PstI site found at 1964 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Our project aims to reduce the carbon emission during the fermentation of Clostridium tyrobutyricum (C. tyrobutyricum) by constructing NOG pathway to integrate with the native EMP pathway of the strain. NOG pathway is an artificial pathway and functions as a fructose-6-phosphate (F6P) shunt. Through carbon rearrangement, this pathway can convert 1mol of F6P to 3mol of AcP without any loss of carbon. By comparison, we found that most of the key enzymes in NOG pathway natively exist in C. tyrobutyricum and the functions of the absent key enzymes can be carried out by phosphoketolases (Figure 1). Therefore, in order to construct NOG pathway in C. tyrobutyricum L319, we introduced phosphoketolase (F/Xpk) gene into the strain. We designed this ancestral F/Xpk sequence in order to engineer a C. tyrobutyricum strain with better fermentation performance, more efficient carbon conservation and less carbon emission.
Source
This part was the ancestral sequence predicted by FireProt-ASR (https://loschmidt.chemi.muni.cz/fireprotasr/) based on FXpk sequence (BBa_K4119076).