Regulatory

Part:BBa_K4886005:Design

Designed by: Leyu Xu   Group: iGEM23_Nanjing-BioX   (2023-09-17)


Ptkt


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Our project aims to construct NOG pathway in C. tyrobutyricum L319 by introducing the phosphoketolase (F/Xpk) gene into the strain. Ptkt is a native transcriptional promoter in C. tyrobutyricum L319. We used this promoter to test if it can provide a robust expression in the strain. We also tested function of Ptkt promoter in the expression of Bs2 gene in C. tyrobutyricum.

Source

The source of the part will be its sequence which was retrieved from NCBI.

References