Part:BBa_K4885001:Design
bcd,butyryl-CoA dehydrogenase coding sequence
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 181
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 310
Design Notes
In C. tyrobutyricum L319, glucose is converted to acetyl-CoA which is then mainly converted to butyryl-CoA by the following enzymes: thiolase (thl), 3-hydroxybutyryl-CoA dehydrogenase (hbd), crotonase (crt), and butyryl-CoA dehydrogenase (bcd). Butyryl-CoA is subsequently converted to butyrate in the native strain. In the preliminary experiment, we discovered that the yield of butyrate and butanol increased efficiently through the overexpression of bcd and crt. Therefore, crotonase and butyryl-CoA dehydrogenase are considered as rate-limiting enzymes. We used the endogenous bcd and crt genes and Pthl promoter from C. tyrobutyricum L319 to construct the part of Pthl-bcd-crt to overexpress crt and bcd in C. tyrobutyricum to enhance the butyrate and butanol synthesis pathway.
Source
The part sequence is obtained by mutating two sites from BBa_K4119027