Part:BBa_K4882003

SuperNova-SV40 NLS

This is a red fluorescent protein SuperNova fused with an SV40 nuclear localization signal (NLS).

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 714
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 151
  Illegal BsaI.rc site found at 442
  

Usage and Biology

Metarhizium anisopliae is an entomopathogenic fungus widely used as a biopesticide. SuperNova (BBa_K4882008) is a red fluorescent protein that produces reactive oxygen species (ROS) in the presence of green-yellow light (~579 nm). To improve the safety of M. anisopliae, SuperNova is introduced as a part of a suicide switch. The SV40 NLS (BBa_K2549054) should guide the protein to be transported into the cell nucleus, and let ROS attack the most vulnerable genomic DNA (Paardekooper et al., 2019).

References

Paardekooper LM, van Vroonhoven E, Ter Beest M, van den Bogaart G. Radical Stress Is More Cytotoxic in the Nucleus than in Other Organelles. Int J Mol Sci. 2019 Aug 25;20(17):4147. doi: 10.3390/ijms20174147. PMID: 31450682; PMCID: PMC6747261.