Device
Part:BBa_K4881027:Design
Designed by: Nyahna Sanchez Group: iGEM23_FDR-HBPeru (2023-10-06)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 1828
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1027
Illegal AgeI site found at 247
Illegal AgeI site found at 364 - 1000COMPATIBLE WITH RFC[1000]
References
Knott, B. C., Erickson, E., Allen, M. D., Gado, J. E., Graham, R., Kearns, F. L., Pardo, I., Topuzlu, E., Anderson, J. J., Austin, H. P., Dominick, G., Johnson, C. W., Rorrer, N. A., Szostkiewicz, C. J., Copié, V., Payne, C. M., Woodcock, H. L., Donohoe, B. S., Beckham, G. T., & McGeehan, J. (2020). Characterization and engineering of a two-enzyme system for plastics depolymerization. Proceedings of the National Academy of Sciences of the United States of America, 117(41), 25476–25485. https://doi.org/10.1073/pnas.2006753117
Sources
MHETase and PETase plasmid on Addgene: pCJ190 (Plasmid #162667)