Coding

Part:BBa_K4880008:Design

Designed by: Xinran Xia   Group: iGEM23_BASIS-China   (2023-09-01)


sepA1


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 69
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 69
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 69
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 81
    Illegal SapI.rc site found at 55


Design Notes

-


Source

This sequence is derived from derived from the large plasmid pANL of Synechococcus 7942.


References

Zhou, Y., Sun, T., Chen, Z., Song, X., Chen, L., & Zhang, W. (2019). Development of a new biocontainment strategy in model cyanobacterium synechococcus strains. ACS Synthetic Biology, 8(11), 2576–2584. https://doi.org/10.1021/acssynbio.9b00282