Translational_Unit

Part:BBa_K4879008:Design

Designed by: Rohaneshwar Manikandan   Group: iGEM23_IISER-Pune-India   (2023-10-08)


CvFAP Expression Construct


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 2320
    Illegal suffix found in sequence at 2590
    Illegal EcoRI site found at 2584
    Illegal PstI site found at 790
    Illegal PstI site found at 1222
    Illegal PstI site found at 1489
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2320
    Illegal EcoRI site found at 2584
    Illegal SpeI site found at 2591
    Illegal PstI site found at 790
    Illegal PstI site found at 1222
    Illegal PstI site found at 1489
    Illegal PstI site found at 2605
    Illegal NotI site found at 2326
    Illegal NotI site found at 2598
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2320
    Illegal EcoRI site found at 2584
    Illegal XhoI site found at 1955
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 2320
    Illegal suffix found in sequence at 2591
    Illegal EcoRI site found at 2584
    Illegal PstI site found at 790
    Illegal PstI site found at 1222
    Illegal PstI site found at 1489
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 2320
    Illegal EcoRI site found at 2584
    Illegal XbaI site found at 2335
    Illegal SpeI site found at 2591
    Illegal PstI site found at 790
    Illegal PstI site found at 1222
    Illegal PstI site found at 1489
    Illegal PstI site found at 2605
    Illegal NgoMIV site found at 2110
    Illegal AgeI site found at 2086
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The expression cassette contains overhangs on both its 5' and 3' ends that are complementary to the JcFATB and JcFATA expression cassettes respectively in order to facilitate DNA assembly with the NEBuilder HiFi DNA Assembly kit.


Source

The expression construct was synthesized by Twist Biosciences.

References