Translational_Unit

Part:BBa_K4879006:Design

Designed by: Rohaneshwar Manikandan   Group: iGEM23_IISER-Pune-India   (2023-10-08)


JcFATA expression construct


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1642
    Illegal suffix found in sequence at 1912
    Illegal EcoRI site found at 1906
    Illegal PstI site found at 862
    Illegal PstI site found at 1469
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1642
    Illegal EcoRI site found at 1906
    Illegal SpeI site found at 1913
    Illegal PstI site found at 862
    Illegal PstI site found at 1469
    Illegal PstI site found at 1927
    Illegal NotI site found at 1648
    Illegal NotI site found at 1920
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1642
    Illegal EcoRI site found at 1906
    Illegal BglII site found at 1311
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1642
    Illegal suffix found in sequence at 1913
    Illegal EcoRI site found at 1906
    Illegal PstI site found at 862
    Illegal PstI site found at 1469
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1642
    Illegal EcoRI site found at 1906
    Illegal XbaI site found at 1657
    Illegal SpeI site found at 1913
    Illegal PstI site found at 862
    Illegal PstI site found at 1469
    Illegal PstI site found at 1927
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The expression cassette contains overhangs on both its 5' and 3' ends that are complementary to the CvFAP expression cassette and the pUC19 plasmid respectively in order to facilitate DNA assembly with the NEBuilder HiFi DNA Assembly kit.


Source

The expression construct was synthesized by Twist Biosciences.

References