Coding

Part:BBa_K4879002:Design

Designed by: Rohaneshwar Manikandan   Group: iGEM23_IISER-Pune-India   (2023-10-08)


CvFAP


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 259
    Illegal PstI site found at 691
    Illegal PstI site found at 958
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 259
    Illegal PstI site found at 691
    Illegal PstI site found at 958
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1424
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 259
    Illegal PstI site found at 691
    Illegal PstI site found at 958
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 259
    Illegal PstI site found at 691
    Illegal PstI site found at 958
    Illegal NgoMIV site found at 1579
    Illegal AgeI site found at 1555
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The coding sequence has been codon-optimized for Y. lipolytica using SnapGene. It also has silent mutations to make the part Type II Standard compatible.


Source

The part had been synthesized by Twist Bioscience, iGEM's DNA synthesis partner.

The truncated CvFAP cDNA sequence was provided in the supplementary information of the article attached in the references.


References

Bruder, S., Moldenhauer, E.J., Lemke, R.D. et al. Drop-in biofuel production using fatty acid photodecarboxylase from Chlorella variabilis in the oleaginous yeast Yarrowia lipolytica. Biotechnol Biofuels 12, 202 (2019). https://doi.org/10.1186/s13068-019-1542-4